PJB-2022-446
Bioremediation of ore tailing by Aspergillus niger for producing biosynthesized nano fertilizers as plant growth stimulator
Sobia anjum
Abstract
Ore Tailing, a rich source of minerals stimulates the researchers to microbially recover valuable metals from this toxic waste to usable for in an eco-friendly manner. In current research, A. niger metabolites exhibited good potential for metal ions solubilization and further conversion into nano formulation by protein end capping. Synthesis of biorecovered nanoparticles were confirmed by systematic characterization using AAS, UV/VIS, FTIR, SEM and Zetasizer analysis. These BNPs with various concentrations (0.2, 0.4, 0.6, 0.8 and 1.00 ppm) were evaluated for their efficacy on seed germination and seedling growth of wheat (Triticum aestivum L.) by seed priming technique. Seedlings displayed pronounced seed germination (100 % ± 0.00) and seedling growth in terms of root and shoot length (8.31 ± 0.18 &7.46 ± 0.15) in the seeds treated with 1.00 ppm of BNPs followed by green synthesized Fe NPs and bulk Fe with germination % (99.64 ± 0.12 & 99.25 ± 0.13) and root and shoot length (7.42 & 6.48cm & 6.62 & 6.26cm) respectively. Minimum growth was observed in water as control representing germination % (96.48 ± 0.13) and seedling growth (4.36 & 4.81 cm). BNP has stimulatory effect on wheat seeds as progressive increase in their germination and physical parameters were observed. So, biosynthesized nanoparticles could be an initiative for the development of nano-biofertilizers of potential importance in agriculture sector.
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