PJB-2022-439
Hydrogen sulfide induced by hydrogen peroxide mediates strigolactones-triggered stomatal closure in Arabidopsis thaliana
Yinli Ma, Liping Huang, Zhan Zhang, Shuangshuang Liang and Liuxi Wang
Abstract
The function of hydrogen sulfide (H2S) and interaction with hydrogen peroxide (H2O2) during stomatal movement caused by strigolactones (SLs) in Arabidopsis thaliana were examined. SL triggered stomatal closure and induced rise in H2S content, L-cysteine desulfhydrase (L-/D-CDes) and D-cysteine desulfhydrase (D-CDes) activity increase in leaves of the wild type, but these effects were prohibited by H2S scavenger or synthesis inhibitors. However, SL couldn’t significantly change stomatal aperture in Atl-cdes and Atd-cdes mutants. Moreover, H2O2 scavengers and synthesis inhibitor obviously prohibited SL-caused H2O2 production and stomatal closure of the wild type, but SL couldn’t close stomata and couldn’t cause H2O2 production of guard cells in AtrbohD, AtrbohF and AtrbohD/F mutants. Furthermore, H2O2 scavengers and synthesis inhibitor prevented SL-caused H2S synthesis, L-CDes and D-CDes activity increase in leaves of the wild type, but H2S scavenger or synthesis inhibitors couldn’t inhibit SL-caused H2O2 synthesis. SL increased H2O2 levels in Atl-cdes and Atd-cdes mutants’ guard cells. However, SL couldn’t cause H2S synthesis, L-CDes and D-CDes activity increase in leaves of AtrbohD, AtrbohF and AtrbohD/F mutants. All the data suggested that SLs-caused stomatal closure required H2O2 synthesis derived from NADPH oxidase and subsequent L-/D-CDes-sourced H2S production
To Cite this article:
Ma, Y., L. Huang, Z. Zhang, S. Liang and L. Wang. 2024. Hydrogen sulfide induced by hydrogen peroxide mediates strigolactones-triggered stomatal closure in Arabidopsis thaliana. Pak. J. Bot., 56(2): DOI: http://dx.doi.org/10.30848/PJB2024-2(33)
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