PJB-2022-437
EXTRACTION AND CHARACTERIZATION OF POLYHYDROXYALKANOTES FROM TANNERY WASTEWATER AND AGRICULTURAL SOIL
MARIAM ZAMEER
Abstract
Polyhydroxyalkanotes (PHA) are eco-friendly biopolymers that are used as an alternative to petroleum-based plastics. Wastewater and agricultural soil are cheap and powerful sources of PHA production. In the present study, samples were collected from DADA enterprises Qasoor and agriculture soil of Punjab University Lahore to screen PHA-producing bacteria. Potato Dextrose Agar (PDA) medium supplemented with Nile blue and Nile red was used for screening of PHA producers while bioplastic granules were screened using Sudan black staining. Three PHA-producing bacterial strains (SS8, SS10, and SW9) were screened, purified, and characterized morphologically, biochemically as well as physiologically. The genomic DNA of these strains was sequenced. Phylogenetically, PHA-producing bacteria were identified as Acinetobacter venetianus (SS8), Bacillus pumilus (SW9), and Bacillus pumilus (SS10). Growth kinetics of PHA producers were observed at different time intervals with different carbon sources like 2% glucose, 2% glycerol, 0.2% glycerol, and wastewater. The highest amount of PHA was extracted from strain SS10 i.e., 69.2% in PDA with 0.2% glycerol followed by 66% in PDA with 2% glucose as a carbon source. The other two strains also produced a sufficient amount of PHA. FTIR analysis of the extracted product was performed which showed the presence of a carbonyl (C=O) ester bond. Peaks were observed at 1721, 1722, and 1722cm-1. The study signifies the extraction of biodegradable polymers that decrease the burden on petroleum oil and helps create a cleaner environment.
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