PJB-2022-415
INVESTIGATION OF ANTIBACTERIAL ACTIVITY OF EXTRACTS OBTAINED FROM HYPERICUM PERFORATUM (ST. JOHN'S WORT) PLANT AGAINST GRAM POSITIVE BACTERIA
ÖZGÜL KISA
Abstract
The aim of this study was to determine the antibacterial activity of H. perforatum L. aerial part extracts from two different locations in Turkey. The plant materials were collected from West Anatolian region-Didyma/Aydın (sample A) and East Anatolian region-Tunceli (sample B). The plant materials were macerated with hexane, ethyl acetate, and methanol (MeOH), respectively. MeOH extract from Didyma sample was fractionated with liquid-liquid chromatography using hexane, ethyl acetate, butanol (BuOH: H2O, 70:30) and water as eluents and four fractions were obtained. Antibacterial activity of plant extracts was observed against 99 Gram positive clinical isolates. Four different standard strains (Staphylococcus aureus ATCC 29213, Enterococcus faecalis 29212, Staphylococcus epidermidis ATCC 12228, Bacillus cereus ATCC 14579) were used as quality control strains. The antibacterial effects of all extracts were evaluated by using the broth microdilution test. Two-fold dilutions of the extracts were subsequently made to obtain the serial dilutions (3.125-3200 µg/mL) of the extracts in 96 well plate. All of extracts exhibited inhibitory activity against both of clinical isolates and standard strains at different concentrations. Significant difference between the Sample A extracts and Sample B extracts were not found. Ethyl acetate and hexane extracts of Sample A were the more effective against all Gram-positive bacteria with MIC50 concentrations of 6.125 – 12.5 μg/mL (ethyl acetate extract) and 6.125 - 1 μg/mL (hexane extract). The extracts of H. perforatum may be a good therapeutic option for Gram positive pathogens.
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