PJB-2022-411
Study on the Bud-Formation-Related MADS-Box Genes in Cerasus xueluoensis
Tong Wu
Abstract
The transcriptome of axillary bud meristematic tissues from Cerasus xueluoensis was sequenced, and the relevant gene information was analyzed, after which it was screened and two MADS-box genes relevant to the specific flower bud differentiation of C. xueluoensis were cloned; the functions of the two genes in the bud differentiation of C. xueluoensis were explored by a homology comparison and phylogenetic analysis, and the protein properties as well as expression characteristics of both were analyzed. CxlMADS1 and CxlMADS2 were cloned by the RACE technique, and a bioinformatics analysis showed that the full lengths of the CxlMADS1 and CxlMADS2 genes were 957 bp and 1274 bp, while the ORFs were 636 bp and 708 bp, encoding 212 and 236 amino acids, respectively. The homology comparison analysis showed that CxlMADS1 was the closest relative to the AGL6 gene, so it was classified as an AGL6-like gene. CxlMADS2 had the highest similarity to the Prunus pseudocerasus dormancy-associated gene DAM3, so CxlMADS2 was classified as a DAM3 lineage, and the DAM gene is an SVP homolog. Gene expression analyses of different tissues and organs showed that the CxlMADS1 gene was expressed merely in four-wheeled floral organs, while the CxlMADS2 gene was expressed in both leaves and four-wheeled floral organs. The cloned CxlMADS1 and CxlMADS2 genes obtained may be related to the differences leading to the differentiation time and rate of the flower bud differentiation of C. xueluoensis, especially the fast and slow differentiation of the three at the sepal differentiation stage, which laid the foundation for the subsequent verification of the gene function and is important for understanding the flower bud differentiation of the plant.
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