PJB-2022-387
GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES FROM SELENICEREUS GRANDIFLORUS EXTRACT AND ITS ACTIVITY AGAINST ROOT KNOT NEMATODE MELOIDOGYNE INCOGNITA
Sidra Ahmad
Abstract
Meloidogyne incognita is an economically important plant parasitic nematode which causes severe damage to a wide variety of crops mainly vegetable crops. The current study was aimed at studying the phytochemical composition of Selenicereus grandiflorus, synthesis of zinc oxide nanoparticles and evaluate the effect of plant extract and zinc oxide nanoparticles of S. grandiflorus against Meloidogyne incognita. Qualitative phytochemical analyses revealed the presence of various phytochemical constituents in plant extract of S. grandiflorus. Zinc oxide nanoparticles were synthesized from S. grandiflorus extract and then characterized by UV-vis spectroscopy, XRD (X-Ray Diffraction), SEM (Scanning Electron Microscope) and EDX (Energy Dispersive X-ray Spectroscopy). Plant extract of S. grandiflorus and its zinc oxide nanoparticles showed significant nematicidal activity against Meloidogyne incognita. Zinc oxide nanoparticles showed enhanced activity as compare to plant extract. After 24 hours, highest dose (1000 µg/ml) of nanoparticles showed highest mortality i.e 88.5 % while at lowest concentration (100 µg/ml) the mortality was 25.8 %. Likewise, at 48 hours, 1000 µg/ml/ml of nanoparticles showed highest mortality i.e 96.3 % while at lowest concentration the mortality was 33.5 %. At highest time period (72 hours), mortality was highest i.e 99 % at 1000 µg/ml of nanoparticles while lowest dose of nanoparticles also showed good activity which was 87.83 %. By increasing time period and concentration of nanoparticles, mortality of Meloidogyne incognita juveniles was increased. The results from the experiments suggests that in future these nanoparticles can be used as potential bionematicidal agent and good alternative of chemically synthesized nematicides for control of plant parasitic nematodes.
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