PJB-2022-356
Influence of various growth modulators on the rooting, enzymatic activity, and nutrient content of Catalpa bignonioides (Bignoniaceae)
Jiajia Sun, Shuwen Bi, Shanshan Jin, Yange Wang, Hanlei Chen, Ni Ruoyi, Mingyue Ma and Jin’e Quan
Abstract
Background: Ecosystem restoration is a major effort to promote sustainable human development. As a green tree species, the role of the American wood bean tree in promoting the industrialization of seedlings and the strength of scientific and technological innovation cannot be ignored, but the introduction of the American wood bean tree propagation and cultivation system is still immature. The goals of this investigation were to improve the survival rate and rooting rate (RR) of Catalpa bignonioides cuttings; to screen out the optimal formula and processing method; and to offer a theoretical basis and technical direction for further improving the prompt reproduction and widespread administration of C. bignonioides. Using fresh softwood of a 3-year-old parent C. bignonioides as material, three-factor orthogonal tests involving three growth regulator types (indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and rhizogenic powder-1 (ABT-1)), growth regulator concentrations (500, 1000 and 1 500 mg·L-1), and growth regulator action times (10 min, 30 min, and 60 min) were performed to analyze the influence of these three factors on the rooting index during the rooting of cuttings and to determine the MDA content, related enzymatic activity, and changes in soluble sugar (SS) and soluble protein (SP) levels of cutting phloem. The order of influence of the 3 kinds of growth regulators on C. bignonioides was IBA>ABT-1>IAA>contral (CK). Different growth regulator concentrations had significant effects on the RR of C. bignonioides cuttings. Cutting with a processing time of 60 min had the highest RR, callus production rate, and average root number. Among the 3 main factors, growth regulator had the greatest influence on rooting, with each index reaching a significant level. Overall, the malondialdehyde (MDA) levels of cutting treatments were drastically reduced, compared to the CK; the peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD) activity was markedly elevated, relative to CK, and the SS and SP levels were also elevated, compared to the CK overall. Among the three growth regulators, IBA exposure had the most significant impact on the cutting effect, and the rooting effect of 1000mg · L-1 treatment was the best of 60 min was the best for the indicators of the rooting of cuttings. T5 (IBA+1000 mg·L-1+60 min) was the optimal treatment combination, carrying a RR of 84%. Treatments with different growth regulators can reduce the MDA content and increase the related enzymatic activity and nutrient content in cuttings, thus promoting the rooting of cuttings
To Cite this article:
Sun, J., S. Bi, Sh. Jin, Y. Wang, H. Chen, N. Ruoyi, M. Ma and J. Quan. 2024. Influence of various growth modulators on the rooting, enzymatic activity, and nutrient content of Catalpa bignonioides (Bignoniaceae). Pak. J. Bot., 56(1): DOI: http://dx.doi.org/10.30848/PJB2024-1(15)
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