PJB-2022-353
Micropropagation protocol for shoot proliferation of pomegranate via nodal segments
plosha khanum
Abstract
A reliable and optimized micro-propagated true to type protocol for pomegranate has been developed via nodal segments. Pomegranate (Punicagranatum L.) has a paramount significance due to its nutritional and medicinal value as it has been used in maintaining a level of blood cholesterol and treating cancer and many other chronic diseases i.e. Diabetes Mellitus, bronchitis and gastro-intestinal ailments. Pomegranates were traditionally vegetatively propagated but (with the advancement in technology) nowadays, micropropagation is used for their cultivation. Production of true-to-type plants with mass multiplication achieved through micropropagation by overcoming insects, pest and viral/fungal diseases. Two pomegranate varieties: Bhagwa and Pope, were sown in three replications and twelve treatments following the factorial under complete randomized design (CRD) in laboratory. The best explant observed was nodal segments for the establishment of pomegranate culture. The explants were treated with 0.1 and 0.5 % HgCl2 for 5, 10 and 15 minutes, out of which 10 minutes sterilization was considered best treatment. Treatments including (KIN 0.5 mg/l + BAP 0.5 mg/l) as well as (KIN 1 mg/l + BAP 2 mg/l) studied best for the shoot sprouting while at 0.5 to 1 mg/l IAA + 3 mg/l BAP maximum shoot proliferation was observed.
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