PJB-2022-342
Cloning of Bacillus megaterium laccase gene isolated from textile waste water replenished soil
Zannara Mustafa, Ikram Ul Haq, Muhamamd Nauman Aftab, Ali Nawaz, Aziz Ur Rehman and Fatima Akram
Abstract
In the present study, 180 soil samples were collected from various sites contaminated with dye-containing waste to isolate laccase producing bacteria. On nutrient agar containing 0.001% (w/v) copper sulfate and 2 mM syringe filtered guaiacol, 41 bacterial isolates produced a brown zone indicating laccase production. A bigger brown zone of oxidation producing 5 bacterial isolates were selected to find out the one with the highest laccase production. After submerged fermentation, a strain gave 0.18 U/mL activity with 50 mM guaiacol which was the highest among all laccase producers. The 16S rRNA sequencing showed that this maximum laccase producing bacteria was Bacillus megaterium. Its laccase gene was isolated and cloned into pET 21a and transformed into BL21 codon + where after enzyme assay with 50 mM guaiacol it gave 0.31 U/mL activity which depicted a successful increase in the enzyme activity
To Cite this article:
Mustafa, Z., I.U. Haq, M.N. Aftab, A. Nawaz, A.U. Rehman and F. Akram. 2024. Cloning of Bacillus megaterium laccase gene isolated from textile waste water replenished soil. Pak. J. Bot., 56(2): DOI: http://dx.doi.org/10.30848/PJB2024-2(18)
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