PJB-2022-301
FACTOR AFFECTING CALLUS INDUCTION FROM ANTHER AND OVARY OF OKRA (ABELMOSCHUS ESCULENTUS L.).
Ahmed Mahmood Ibrahim
Abstract
The current study examines the callogenesis response of the anther and ovary of okra. As a pre-requisite for the production of haploid progeny via plant tissue culture, the present study was conducted to investigate the effect of various factors, including plant growth regulators (PGR), sucrose concentration, cold treatment, type of media, and culture conditions, on callus induction from anther and ovary culture in Okra 989 hybrid. Flower buds at various stages of microspore and ovule development were sterilised, then successfully removed from the flowers and subjected to different cool pretreatments before being inoculated into media containing various combinations of PGR: NAA, IAA, 2,4-D, BAP, 2iP, Kinetin, Zeatin and TDZ then incubated in the dark and light conditions. The ideal callus induction frequency was 90.0, 67.5, and 55.0% of anther, ovary, and ovule in the optimised MS medium supplemented with 0.5 mg l-1 TDZ + 2.0 mg l-1 NAA when the microspores were in the uninucleate stage and the anther was approximately 7±1 mm long collected. The flower buds for the ovary and ovule, which were harvested one or two days prior to anthesis, measured about 40±2 mm in length.
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