PJB-2022-264
Evaluation of Vitek2 gram-negative identification cards (GN2 card) as a tool for the identification of some gram-negative bacteria isolated from diseased tomato plants from some regions of Kurdistan Region/ Iraq
Kamalaldeen M. Fatah
Abstract
In any pathogen detection and survey program, time preservation and accuracy of microbial identification are essential features for any detection technique in order to be selected and approved. In classical methods morphological and growth features of the pathogens obtained through subjecting them to a number of diagnostic tests such as, Gram staining and microscopy, biochemical reaction, and indicator plants hypersensitivity tests. In the last few decades a number of alternative techniques have been developed as diagnostic tools which were primarily and mainly designed to be used with clinically important human or animal pathogens, but they were later on approved to be used also for the identification of plant pathogens. In this study, which we conducted during the period from July 2019 to July 2021, we use DNA based molecular assay, and conventional biochemical tests to identify Gram negative bacteria isolated from diseased tomato plants. These bacteria then were identified again using the Vitek2 Gram negative identification card technique (GN2 card), which have been developed very recently in the immediate past by BioMérieux/ France specifically for the identification of clinically important Gram negative bacteria. In this study microscopic examination of 50 bacterial isolates shows that these isolates include both Gram positive and Gram negative bacteria, some of which were bacillus and others were cococcus. Ten Gram negative bacteria isolate including the three phytopathogenic bacteria were subjected to DNA based molecular and biochemical identification, results revealed that the pathogenic isolates were identified as Xanthomonas performance, Pseudomonas sp., and Ralstonia sp., All these ten isolates were then subjected to identification using Vitek2 gram negative identification cards (GN2 card), the results of GN2 cards show that this technique either did not identify the bacteria or misidentify them. According to our results we conclude that GN2 card technique is inappropriate and cannot be used for the identification of plant pathogenic bacteria, and there is a need to improve the database stored in the analyzer so that it can identify phytopathogenic Gram negative bacteria.
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