PJB-2022-260
Polarity-Dependent Response of Phytochemical Extraction and Antioxidant Potential of Different Parts of Alcea Rosea
Haq Nawaz
Abstract
In the present study, the polarity-dependent response of extract yield, phytochemical content, and antioxidant activities of the stem, seed, and leaves of Alcea rosea was studied. The powdered samples of the selected parts A. rosea were extracted in solvents of varying dipole moments (hexane: 0 D, ethyl acetate: 2.8 D, and methanol: 5.1 D). The extracts were screened for the presence of important phytochemicals and analyzed for their phytochemical content and antioxidant potential. The studied parts of A. rosea consisted of flavonoids, tannins, terpenoids, saponins, and cardiac glycosides. The regression analysis showed polarity-dependent significant positive effects (p<0.05) on the extract yield, phenolic content, and antioxidant activity in terms of Trolox equivalent total antioxidant activity, ferric reducing power, and nitrogen free radical scavenging activity of the extracts. However, a mixed response of total flavonoids and total tannins content and hydroxyl radical scavenging activity of the extracts was observed against solvent polarity. The study suggests that the polar solvents are more suitable to achieve a good extract yield of phytochemicals possessing a strong antioxidant ability and the solvents of medium polarity may be suitable for the extraction of flavonoids and tannins from the studied parts of A. rosea.
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