PJB-2022-255
Evaluation of Therapeutic Safety of Active Fraction of Lavandula Stoechas in Albino Mice
Aamir Mushtaq
Abstract
This study is the continuation of the previously reported study in which two principle components (phenethylamine and α-tocopherol) of L. stoechas were declared responsible for the nootropic potential. The objective of this study was to find the safety of active fraction of Lavandula stoechas (AfL.s) in albino mice. Acute toxicity study was performed in mice by oral administration of AfL.s in doses 350 and 300 mg/Kg and signs of toxicity were observed for 72 hours. For chronic studies, two groups of animals were administered with 10 ml/Kg P.O normal saline and AfL.s 18 mg/Kg P.O, respectively, daily for consecutive three months. At the end of 90th day, the blood was collected from the heart of mice to perform hematological and biochemical studies and animals were sacrificed to get the brain and liver for histopathological studies. The results indicated that the acute toxic doses of AfL.s produced hyper excitation, convulsions, ataxia, muscle spasm and blanching in mice. Chronic toxicity study indicated that AfL.s (18 mg/Kg P.O) produced non-significant (P ≥ 0.05) changes in hematology (RBCs, platelet, hemoglobin) and serum biochemistry (cholesterol, creatinine, glucose, aspartate transaminase (AST), alanine aminotransferase (ALT) and total bilirubin (TB) of mice. Moreover, the WBCs, hematocrit and alkaline phosphatase (ALP) levels were slightly increased along with significant (P ≤ 0.05) weight reduction in treated mice. No sign of toxicity was observed by hitopathocological studies. Thus, AfL.s is declared safe for long term use at proposed oral dose of 18 mg/Kg P.O in mice. Key words: L stoechas, Acute Toxicity, Chronic Toxicity, AST, ALT,
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