PJB-2022-242
GROWTH DYNAMICS AND SUSCEPTIBILITY PATTERN OF AFLATOXIN PRODUCING ASPERGILLUS FLAVUS TO PLANT ESSENTIAL OILS
Saba Sana
Abstract
Aflatoxins are toxic secondary metabolites hamper food quality and predispose human, animals and birds to multiple clinical manifestations. Feed contaminated with Aflatoxin producing Aspergillus flavus is a source of toxin transmission to animals and birds. It is also a public health concern. Poultry feed analysis for fungal contamination revealed 39 Aspergillus isolates out of which 22 were identified as A. flavus. Highly specific PCR confirmed 12 isolates as A .flavus. Out of which 08 were detected positive for aflatoxin producing and declared as toxigenic. In Principal component analysis (Temperature, pH, Substrates; wheat, maize and rice each with different concentration 1, 3 & 5%) of toxigenic A. flavus (n=08), the highest mean biomass (8.84±0.02g) was produced by A. flavus-cpf15.1 in Sabouraud’s dextrose broth (pH 6) on incubation temperature 22℃. Plant’s essential oils of Eucalyptus globulus, Syzygium aromaticum, and Elettaria cardamomum were assessed for their antifungal potential against aflatoxin producing A. flavus by agar well diffusion and mean ZOI were in ranged from of 31.00±1.00 to 12.67±0.57 mm. In Micro-broth dilution method Minimum inhibitory concentrations (mean MICs) were 1.30±0.45, 0.65± 0.22 and 2.60±0.90 µL /mL for E. globulus, S. aromaticum, and E. cardamomum respectively. It was concluded that toxigenic A. flavus is a common contaminant of poultry feed and feed is a source of mycotoxin transmission to birds. So, among mitigation strategies use of essential oils as feed preservatives is a good choice as these oils have potential to inhibit growth of aflatoxin producing A. flavus.
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