PJB-2022-241
HIGH-DENSITY GENETIC MAP CONSTRUCTION AND QTL MAPPING IN SORGHUM-SUNDANGRASS RIL POPULATION (M81×Sa)
YANLONG LIU
Abstract
Sorghum-sudangrass, a hybrid of sorghum and sudangrass that has the characteristics of high productivity, strong adaptability, and superior quality, is widely used in animal husbandry and aquaculture. Understanding the genetic basis for major agronomic traits is essential for forage sorghum and sudangrass hybrid breeding. In this study, we generated a recombinant inbred line (RILs) derived from a cross between sweet sorghum M81 and sudangrass Sa that composed of 174 RIL individuals. Single-nucleotide polymorphisms (SNPs) derived from genotyping by Restriction-site Associated DNA Sequence (RAD-seq) of RILs were used to construct a high-density genetic map comprising 790 SNPs spanning 2101 cM and coverage of all 10 chromosomes was greater than 80%. Inclusive Composite Interval Mapping (ICIM) was carried out to map quantitative trait loci (QTL) for five forage traits. A total 18 QTLs were identified for plant height (PH, 4 QTLs), fresh weight per plant (FW, 8 QTLs), dry weight per plant (DW, 3 QTLs), stem diameter (SD, 2 QTLs), and sugar content (SC, 1 QTL). In addition, two overlapping QTLs (qFW9.2/qDW9 and qFW10/qDW10.2) were detected for FW and DW. Together, the RIL population and high-density linkage map in this study will be useful for dissecting important traits and provide a valuable resource for future breeding improvement of the forage quality in sorghum-sudangrass hybrids.
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