PJB-2022-228
IN VITRO REGENERATION OF STRIPE EBONY (DIOSPYROS FRUTESCENS BLUME)
Nguyen Minh Ty
Abstract
The stripe ebony tree ( Diospyros frutescens Blume ) is considered as a species in danger of extinction due to overexploitation. Therefore, there is an urgent need to regenerate and conserve the species for future generation. In this study micropropagation protocol of Diospyros frutescens Blume were developed using nature zygotic embryos. Zygotic embryos isolated from nature fruits were germinated on MS medium containing 0.4 mg L-1 Kinetin + 1.0 mg L-1 BA. There were (83.11±0.79%) of explants induced shoots after 30 days of culture. The highest number of shoot on 1/2 MS medium supplemented with 2.5 mg L-1 BA + 0.6 mg L-1 IBA was (5.84±0.035) shoots per explant after 45 days of culture. Percentage of shoot produced roots the highest (85.52±0.48%) after 45 days culture on 1/2 MS medium contain 0.8 mg L-1 IBA.
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