PJB-2022-213
ENDOPHYTES OF MARSDENIA TENACISSIMA DIFFERENTIALLY MODULATE HOST PLANT PRODUCTIVITY AND GLYCOSYLTRANSFERASE GENES FOR THE BIOSYNTHETIC PATHWAY OF SAPONINS
LIU CHENGQIN
Abstract
In the treatment of malignant tumors, leucoemia, cystitis, and asthma, Marsdenia tenacissima has a special curative effect. Although a large number of studies have shown that endophyte can promote growth and accumulation of effective components in host plants, while no study had been reported about endophyte boosting the growth and increase saponins of M. tenacissima in sustainable agriculture. The goal of this work was to identify M. tenacissima endophytic bacteria and fungi, as well as describe their plant growth-promoting (PGP) capabilities. 37 kinds of endophytic bacteria and 22 kinds of endophytic fungi were isolated from the roots, stems and leaves of M. tenacissima. Eight endophytes, including four endophytic bacteria (XG13, XG41, XY11, XG352) and four endophytic fungi (ZJ1, ZG7, ZG1, ZG10), were selected to inoculated M. tenacissima. Of these, six endophytes, including XG13, XG41, XY11, ZJ1, ZG1, ZG10, could promote the accumulation of Tenacissoside H, increased by 22.84%, 49.71%, 14.32%, 0.57%, 34.14%, 3.93%, 45.69% ,respectively. Two endophytes, XG13 and ZG10, could promote the accumulation of Tenacissoside I, increased by 4.55% and 17.03% respectively. Four endophytes, XG13, XG41, XY11 and ZG10, could promote the accumulation of Tenacissoside G, increased by 17.48%, 28.07%, 5.50% and 39.02%, respectively. Based on the selected glycosyltransferase genes related to saponins synthesis, the expression of UGT89 was higher in XY11, UGT85A23 and UGT94E5 were higher in XG41, UGT85A24 and UGT72E1 were higher in ZG10, and UGT74F1was higher in XG13 inoculated M. tenacissima plants compared with the CK. Of the eight endophytes, only XG13, XG41, XY11 and ZG10 could promote the growth and accumulation of saponins in M. tenacissima in the meantime. All in all, these results suggest that these endophytes play a special role in regulating the growth of host plants and the production of saponins in M. tenacissima.
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