PJB-2022-200
Effect of plant growth regulators and dark stress on croton (Codiaeum variegatum) callus induction.
sevtap doksöz boncukçu
Abstract
The croton (Codiaeum variegatum) plant, with its magnificent leaf colors, is a beautiful indoor and outdoor ornamental plant as well as its leaf extracts have many medicinal properties. Usually, crotons are propagated vegetatively. This process is slow in response and many parent plant stocks need to be maintained. Micropropagation is an alternative propagation method that can meet its high demand in a relatively short time. Callus culture is a tissue culture method that is used both in micropropagation and in the production of secondary metabolites. The purpose of this study is to determine the effects of different growth plant regulators and dark growth conditions on callus induction of croton (Codiaeum variegatum). MS medium with 3 % sucrose, 0,8 % agar and different combinations of kinetin, benzyl amino purine (BAP) and Naphthalene acetic acid (NAA) was used.The highest callus diameter was measured as 1.0 cm in M9 medium under both dark and light growth conditions. The maintained dark conditions had a significant effect on callus induction in M4 and M9 media. While callus formation was 22.2% in these nutrient media kept in day/night conditions, it was 94.44 % in dark conditions.
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