PJB-2022-192
DIFFERENTIAL EXPRESSION ANALYSIS OF HEAT SHOCK PROTEIN GENE IN COTTON SPP
sara khan
Abstract
Heat shock proteins (HSPs) acts as chaperons upon activation against various abiotic and biotic stress factors. HSPs have been characterized on basis of molecular weights and each member has it different functions. In response to signals, activation of heat shock factor that binds to heat shock elements and regulate the transcription of HSP. The expressional studies of HSP in many different species have been performed but the exact point in signal control still need to be explored. The aim of present study is differential expression of class I HSP17.5 gene in cotton spp. Total RNA was isolated from cotton species G. hirsutum and G. arboreum and cDNA was synthesized for amplification of HSP gene. The GhHSP 17.5 was sequenced and characterized through in silico including different searches, multiple alignment followed by phylogeny study. The three dimension structure was predicted by I-TASSER. The 3D structures assessment and interactions were validated by Ramachandran plot. PCR analysis of HSP 17.5 gene was validated by analysis through cotton functional genomic database. Key words: HSPs, Heat shock factors, Stress response, biotic and abiotic stress.
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