PJB-2022-179
HIGH-DENSITY GENETIC MAP CONSTRUCTION AND QTL MAPPING IN SORGHUM-SUNDANGRASS RIL POPULATION (M81×Sa)
YANLONG LIU
Abstract
Sorghum-sudangrass, a hybrid of sorghum and sudangrass that has the characteristics of high productivity, strong adaptability, and superior quality, is widely used in animal husbandry and aquaculture. In this study, 174 recombinant inbred line (RILs) derived from a cross between sweet sorghum M81 and sudangrass Sa. Single-nucleotide polymorphisms (SNPs) derived from genotyping by Restriction-site Associated DNA Sequence (RAD-seq) of RILs were used to construct a high-density genetic map comprising 790 SNPs spanning 2101 cM. Inclusive Composite Interval Mapping (ICIM) was carried out to map quantitative trait loci (QTL) for five forage traits. A total 18 QTLs were identified for plant height (PH, 4 QTLs), fresh weight per plant (FW, 8 QTLs), dry weight per plant (DW, 3 QTLs), stem diameter (SD, 2 QTLs), and sugar content (SC, 1 QTL). In addition, two overlapping QTLs were detected for FW and DW. The research results showed that a high-density map is dissecting important traits and provide information for the improvement of the forage quality in sorghum-sudangrass hybrids.
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