PJB-2022-173
EXPRESSION OF BABYBOOM GENE (BBM) IN COTTON (GOSSYPIUM HIRSUTUM L.) TO ENHANCE REGENERATION CAPACITY
Allah Bakhsh
Abstract
Over the years regeneration and transformation efficiency of numerous recalcitrant crops have been attempted to reach maximum capacity. These attempts are successful case to case and not universal. The genetic transformation of cotton has its own priorities due to increasing demand of natural fibre. The tissue culture protocols are not satisfying and not giving expected results. Novel approaches such as usage of morphogenic genes in overexpression purposes have promising results and can change the recalcitrant nature of a crop. This current research is attempted and focused on improving regeneration capacity of the Gossypium hirsutum L., cv Coker 312 using novel BBM transcription factor. Moreover, it was tested whether spontaneous somatic embryogenesis will occur. For this reason, 35S:BnBBM and 35S:GmBBM constructs were developed and transformed in cotton using Agrobacterium mediated transformation. Obtained results explain functions of the gene and enlighten processes of SE in cotton during different stages of development. The results give insight into somatic embryogenesis phenomenon of cotton tissue culture and Transformation for the purpose to incorporate traits of economic importance.
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