PJB-2022-118
Expression of Heavy Metal ATPases (HMA1 and HMA3) in Brassica nigra and Brassica juncea Grown at Different Cu Levels
Nuriye Merakli
Abstract
Brassica juncea and Brassica nigra are well-known metal accumulator plant species that can tolerate and accumulate large amounts of metals, including copper, in their shoots. In this research, B. juncea and B. nigra plants were grown in the soil at different Cu concentrations (from 0 to 1000 µM). Plants showed no toxicity symptoms and accumulated a significant amount of metal in the leaves. Cu content in the root and leaves of these plants was analyzed using Atomic Absorption Spectrophotometer (AAS). HMA1 and HMA3 expression levels in the leaves of both plant species were analyzed using R-T qPCR. Data show that HMA1 and HMA3 expression in the leaf samples gradually increased with increased metal levels in plants till 50 μM to 100 μM. Then expression level slowly dropped in the leaves when plants were subjected to high metal concentrations (above 100 μM Cu). Gene expression analysis showed that increased metal levels in the leaves trigger the expression of the HMA1 and HMA3 genes, suggesting that they play an important role in Cu detoxification and sequestration at the subcellular level. These results demonstrate that metal ATPases play a crucial role in Cu tolerance in the plants grown at different Cu levels. The Cu accumulation and tolerance in both plants is probably a multi-genetic response, possibly involving several other transporter genes in the stress signal pathway.
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