PJB-2022-104
In vitro bioactivity and antimicrobial efficacy of modified solvents evaporation assisted R. ellipticus
Samriti Sharma
Abstract
The present investigation has been undertaken to study the antimicrobial and antioxidant properties leaves, fruit and root extracts of Rubus ellipticus. R. ellipticus fruit methanol extract, ethanol leaf extract and root methanol extract showed significantly (<0.05) maximum in vitro antioxidant activity. All tested microorganism was observed significantly (<0.05) for susceptibility against ethanol, methanol, petroleum ether and chloroform extracts of leaves, fruit and root of R.ellipticus. The zone of inhibition shown by methanol extract of leaves and ethanol extract of fruit for Staphylococcus aureus, E. coliO157, Salmonella enterica, Candida albicans, and against E. coliO157 and Candida albicans were significantly (<0.05) 12.0 ± 1 mm, 11.0 ± 1 mm, 9.0 ± 1 mm, 0.8 ± .10 mm and 10.0 ± 1 mm and 0.6 ± 0.10 mm respectively. Whereas, chloroform extract of root and chloroform extract of fruits showed significantly (<0.05) 7 ± 1 mm and 6 ± 1 mm zone of inhibition for Staphylococcus aureus, whereas ethanol extract of leaves showed significantly (<0.05) 13.0 ± 1 mm zone against Trichophyton rubrum. Functional group analysis done with ATR showed maximum availability of alkynes and alkanes in root, fruit and leaves, while HPLC analysis revealed significantly (<0.05) higher gallic acid level of 6.57mg, 22.65mg and 20.88mg as compare to rutin i.e, 9.25mg, 7.85mg and 2.0mg in leaves, fruit and roots of Raspberry, respectively. During the kinetics of time-kill, R. ellipticus extracts of leaves, fruit and root significantly (<0.05) showed that S. aureus highly susceptible as compare to E. coliO157, Salmonella enterica, Candida albicans and Trichophyton rubrum Thus, it was concluded that the raspberry is an important source of phyto-chemicals having antioxidant and antimicrobial activities.
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