PJB-2022-61
The study on genetic diversity and phylogenetic relationship of Malus spp. using chloroplast DNA PCR-RFLP markers
Dechang Hu
Abstract
This study was conducted to reveal genetic diversity among 28 genotypes of 22 Malus species using chloroplast DNA PCR-RFLP markers. Fifty-four cpDNA primer combinations were used in this study. Approximately 16.0kb, 10.0% of the chloroplast genome was analyzed. From the nine universal chloroplast primer pairs, four primer pairs amplified the fragments and their combinations showed visible polymorphic patterns and revealed inter- and intra-specific chloroplast variation. Based on cpDNA PCR-RFLP data, the genetic similarity coefficient were calculated by NTSYS software varied from 0.57 to 1.00. UPGMA cluster analysis showed 4 species of M. asiatica, M. yunnanensis, M. hupehensis and M. sikkimensis were clustering separate, while the other accessions were divided into 3 groups. The genotypes of Sect. Gymnomeles and Sect. Malus had most clustered into one category, but mingled in each subgroup, which suggested that their origin female parents should be the same or had closely relationships. Two species, M. prattii and M. yunnanensis, had completely separated in dendrogram, which indicated that they had different origin female parent. Among four species native to North American region, M. ioensis and M. angustifolia had the same or nearly relationship of the female parent, but far away with M. fusca and M. platycarpa.
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