Paper Details

PJB-2022-41

IN SILICO IDENTIFICATION AND CHARACTERIZATION OF CUMULATIVE ABIOTIC STRESS RESPONDING GENES IN TOMATO (LYCOPERSICUM ESCULENTUM L)

DR. HUMAIRA ABDUL WAHID
Abstract


Abstract Tomato (Lycopersicum esculentum. L) is popular worldwide due to its nutritive value and known as protective food. Economically it is very important vegetable of world. Tomatoes are challenged by number of abiotic and biotic factors reducing their ideal growth, development and production. Expression profiling is very significant tool for the study of organism responds due to abiotic changes. The identification of cumulative abiotic stresses genes, responding to three or four abiotic stresses will be a good source of genes for the production of the abiotic stress resistant food crops. In tomato numerous studies have been done to find out the expression of genes under different abiotic stress conditions, using DNA microarray technology. In this research microarray data for four abiotic stresses salt, heat, cold and drought were analyzed using bioinformatics tools to identify cumulative abiotic stress responding genes. Total 248 cumulative abiotic stress responding genes were identified after the study of 64896 ESTs. The identified genes were also characterized on the basis of their function and validated using Arabidopsis thaliana as reference organism.

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