PJB-2022-3
Characterization of EST-SSR Markers for Genetic Diversity Assessment of Bletilla striata in Guizhou Province
Lin Li
Abstract
To study the genetic diversity and population structure, 25 EST-SSR markers were developed for Bletilla striata (Orchidaceae), an endangered orchid medicinal species in China. These markers were firstly generated from transcriptome data of B. striata by using Illumina paired-end sequencing technology. A total of 108 loci were amplified, in which 91 showed polymorphism among 23 landraces. The alleles ranged from one to eight, with average levels of the observed and expected heterozygosity were 1.345 and 1.840, respectively. And 23 landraces of B. striata were classified into three main clusters by these markers at a genetic similarity coefficient value of 0.74. These newly developed EST-SSR primers facilitated the studies of genetic diversity, phylogenetic and population structure in B. striata. It also indicated that the newly characterized EST-SSR markers may be useful in the exploration of range expansion of B. striata, and in cross-species application for Orchidaceae.
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