PJB-2021-725
Seed biopriming with Phanerochaete chrysosporium Enhances Tolerance of Wheat to Salt Stress through Improvement of TaEXPB23 Expression
Ashraf S.A. El-Sayed
Abstract
Abstract Biopriming of wheat grains with Phanerochaete chrysosporium strongly enhanced salt tolerance of wheat as revealed from the physiological and biochemical parameters, from our previous study. Thus, the main objective this work was to validate the acquired salt resistance of wheat in response to biopriming with P. chrysosporium at the metabolomic and molecular levels. The correlation of phytohormones and molecular expression of TaEXPB23 expansin gene by qRT-PCR of wheat bioprimed with P. chrysosporium comparing to non-bioprimed plant under salt stress was explored. At 100 mM NaCl, the concentrations of IAA, gibberellic acid and KT of P. chrysosporium bioprimed wheat was increased by about 10-12 folds in response to non-bioprimed wheat plants. As well as, the levels of methyl Jasmonate, salicylic acid, and abscisic acid were increased by about 5-7 folds on wheat bioprimed with P. chrysosporium comparing to non-bioprimed plants at 100 mM NaCl. The expression of TaEXPB23 in wheat bioprimed with P. chrysosporium was increased about by 4 folds at 100 mM NaCl after 45 days of treatment comparing to non bioprimed what plants at the same salt concentration and culturing time. So, it could be concluded that, biopriming of wheat with P. chrysosporium significantly increase/upregulates the expression of TaEXPB23 gene, comparing to non-primed plant, ensuring the role of expansin “cell wall loosening proteins†on the plant salt tolerance mechanism.
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