PJB-2021-701
Isolation and characterization of peroxidase P7-like gene and GDP dissociation inhibitor like gene from potential medicinal plants. A step towards more understanding of defence and cell signaling.
Ms UME HABIBA
Abstract
Abstract Defensin genes are the part of plant defence system that get activated whenever the plant is exposed to biotic or abiotic stresses. They play a vital role in controlling many signaling pathways involved in various plant defence mechanisms. This research work was aimed to isolate and characterize novel defensin genes from selected medicinally important plants. DNA of two plants Albizia lebbeck and Moringa oleifera was subjected to PCR amplification using gene specific primers of defensin genes. Two novel defensin genes were isolated from both plants that showed the gene sequence length of 300 bp for A. lebbeck and 150 bp for M. oleifera. Bioinformatic analyses were performed and their orthologous sequences were retrieved and aligned which revealed 100% similarity of A. lebbeck gene with Musa acuminate peroxidase P7-like gene whereas M. oleifera gene showed 85% similarity with Manihot esculenta GDP dissociation inhibitor gene. The reliability of homology models of these sequences was confirmed using programs PROCHECK and ProSA. The results of this study confirmed the presence of novel genes having peroxidase and GDP dissociation inhibitor gene-like activity in A. lebbeck and M. oleifera, respectively with the potential defence role in plants. Therefore, the defensin genes of both plants can serve for the synthesis of transgenic self-defensive plants that will be more disease resistant and can give better crop production in the future.
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