PJB-2021-698
CHEMICAL MUTAGEN EMS AFFECTS AGRONOMIC AND CELL CHARACTERISTICS OF BARLEY (HORDEUM VULGARE L.)
Ali Arminian
Abstract
To study the effects of ethyl methane sulfonate (EMS) mutagen on the agronomic and cellular (cytogenetic) properties of barley, seeds were germinated and cultivated in plastic pots in greenhouse following surface sterilization. Seeds were then treated and cultured under different levels of EMS (0.1, 0.2 and 0.3%). As a result, the mutagen caused chromosomal abnormalities. Increasing of mutagen dose increased the amount of aberrations, as with and the highest occurred under concentration of 0.3% which resulted in metaphase index and significant chromosomal aberrations. Agronomically, there were significant differences between barley lines for numbers of tiller and fertile tiller, plant and stem height, spike length, number of grains per spike, 100 grain wt and stem wt. The most affectability occurred for plant height and total number of seeds. Overall, it is concluded that despite the beneficial effects of EMS, such as mutation breeding, one should not neglect its harmful effects.
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