PJB-2021-678
FATTY ACID PROFILING AND PHYSIOCHEMICAL CHARACTERIZATION OF CHLORELLA SOROKINIANA POTENTIALLY USED FOR BIOFUEL PRODUCTION
Muhammad Ammar
Abstract
Rising oil prices and climate change have resulted in more emphasis on research into renewable biofuels. In this study, different water samples were collected from local vicinities for the isolation of local isolates of microalgae to check their potential towards the production of biofuel by the addition of different chemical substrates. Five different concentrations of ascorbic acid and iron (III) chloride (0 µM, 1 µM, 2.5 µM, 5 µM & 10 µM) are used as substrates. Microscopic analysis evaluated that samples belong to genus Chlorella and further molecular identification revealed that the samples are C. sorokiniana. Among all the concentrations of ascorbic acid 2.5µM is most effective against the C. sorokiniana strain 1 (Safari Wildlife Park, Lahore) and C. sorokiniana strain 2 (Bahria Town, Lahore) while Chlorella sorokiniana Strain 3 (SukhChane Society, Lahore) responded at 2.5µM & 5µM in term of biomass production. FeCl3 (2.5µM) is effective against C. sorokiniana strain 1 while the growth of C. sorokiniana strain 2 and C. sorokiniana Strain 3 is inhibited. Lipid content analysis revealed that only the C. sorokiniana strain 1 shows effective results at 1µM & 2.5µM of ascorbic acid and FeCl3 respectively. Those concentrations which give the significant results of lipid production were preceded for fatty acid profiling. Results indicate that the C. sorokiniana strain 1 can be considered as a source of alpha-linolenic acid; the basic constituent of biofuel production. In this study, we conclude that C. sorokiniana strain 1 is useful for the production of environment friendly biofuel.
To Cite this article:
Download
