PJB-2021-569
FUNCTIONING OF IKU2 GENE CONTROLLING SEED SIZE IN SOYBEAN USING TILLING AND EcoTILLING
Yasir Ahmed Gamar
Abstract
Gene manipulation as mutation has a potential to bring heritable changes, which might be useful forplant breeding implementations.Crop grain yield can be increased through harnessing of genes responsible for seed attributes.IKU2 is a homologous gene of AtIKU, one of 476 putative of LRR-RLK genes were previously identified from the whole genome of soybean.TILLING is a reverse genetic non-transgenic, has power to provide the approximate location within a few base pairs of the induced mutation to reveal variations. EcoTILLING is similar to TILLING was modified to mining for the natural variations. Targeting genes through TILLING allows the development of novel traits and functional markers simultaneously because the mutation serves as landmarks that can be converted into a molecular marker. In this study TILLING and EcoTILLING employed to function and uncover induced and natural variations ofIKU2related to soybean seed size. A total of 1046 (476 EMS treated and 570 untreated) soybean plants were investigated for induced mutation and natural variations. We have developed mutagenized population of soybean using 0.62% EMS mutagen showing mutation density of 1/335 kb. TILLING results showed eight variations, two of them are true EMS induced, express missense and intronic mutations. Two haplotypes were deduced and linked to soybean seed size. Six natural variations were observed through EcoTILLING in untreated population. These natural variations formed three haplotypes linked to the soybean seed size. We have explored nucleotide variations in IKU2 through TILLING and EcoTILLING, which suggested the involvement of the target gene in conferring seed size in soybean.
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