PJB-2021-564
EFFICIENT METHOD FOR MASS PROPAGATION OF ORCHID (vappodes phalaenopsis) USING POLYETHYLENE GLYCOL (PEG) TO STUDY THE INDUCED WATER STRESS ON THE RELATIVE GROWTH OF ORCHID PLANTS
Saniya Jamal
Abstract
Tissue culture is a fascinating tool that allows the rapid production of genetically identical, high yield and disease resistance, long shelf life plants using relatively small amounts of water, space, supplies, and time. Drought stress is a major abiotic constraint limiting the crop production worldwide. Orchid species is considered drought-sensitive species. The present research work involves shoot induction, leaves induction, and callus induction of Orchid species vappodes Phalaenopsis and investigated the effects of drought stress on the in-vitro growth of orchid. Solid Polyethylene glycol (PEG-6000) of high strength (0.5gm/L) was used for drought stress induction in MS. The shoot and leaf induction response from explants (shoot tips) showed that Murashige Skoog (MS), 2ml BAP, and 1 ml of NAA proved best for shoot initiation & PEG 0.5.gm/Liter Murashige Skoog (MS), 2ml BAP, and 1ml of NAA proved best for callus initiation. PEG reduces the transpiration of orchid plants by the reduction in the number of leaves and shoots and increases in the number of calluses. It was vice versa in MS medium without PEG. Keywords: PEG, tissue culturing, drought stress, BAP, NAA.
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