PJB-2021-541
Micropropagation of white crysanthemum (Dendranthema × grandiflorum) through Tissue Culture Technique.
Dr. Syeda Kahkashan Kazmi
Abstract
Chrysanthemum locally known as ‘Gul e Daoudi’ is an ornamental plant used both as cut flower and potted plant for its aesthetic beauty and long lasting presence. A biotechnological susbtitute to produce large quantities of healthy plants is in-vitro micropropagation of healthy explants and meristems into disease free and identical progenies. In this study antral shoot multiplication was attained on optimized MS medium. Proliferation of axillary shoots and growth was obtained from the nodal portion of the mature plants in vitro, exceptionally modified by different concentrations of the BAP. Best response towards highest mean number of shoots proliferation was achieved on ½ MS media along with 4.0mg/l BAP. Highest mean length of shoots was recorded on ½ MS media with 1mg/l BAP. Highest mean number of leaves were produced on ½ MS with 3.0 mg/l BAP. The present research verified for large scale production of ornamental plants with high commercial value on cost effective basis.
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