PJB-2021-530
Molecular Docking study reveals the chemical cues and interactions involved in herbivory induced jasmonate mediated plant defense mechanism
Qudsia Yousafi
Abstract
Plant perception of an insect attack is the first step for defense. Molecules generated by the oral secretion of the insect interact with the plant receptors to trigger plant defense mechanism. In the present study we selected some highly cited insect elicitors molecules, i. e., volicitin, caeliferin and bruchin which interact with plant defense elicitors, located on the plant cell surface, i.e., systemin, inceptin and peps. This interaction activates plant receptors SYR1, LRR, PEPR and trigger downstream defense signaling. The octadecanoid pathways, involving enzymes allene oxide synthase (AOS) and Hydroxyperoxide lyase (HPL) are activated, which interact with 9 and 13- hydroxyperoxides to produce Jasmonic acid (JA) and green leafy volatiles (GLVs) main regulator in plant defense. We docked the elicitors with receptors and enzyme with substrates in the pathway of JA production. Phe was found to be an important amino acid which interacts with 13- hydroxyperoxides in the case of AOS to produce JA but not in the case of HPL. JA is converted to JA-Ile which shows strong binding with COI1 and COI1-JA-Ile complex docked with JAZ which showed strong interaction with five hydrogen and one salt bridge bond. AOS and HPL showed less than 40% identity for sequence and structure alignment. AOS and HPL showed interaction between each other and showed a common interaction partners of Lipoxygenase family. HPL shows interaction with ADH2 (Alcohol dehydrogenase) involved in GLVs production. AOS also showed interaction partner AOC, COI1 and OPR1 which are involved in JA induced plant defense mechanism.
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