PJB-2021-484
Genetic diversity of Elymus dahuricus: morphology and microsatellite markers
Qiang Zhang
Abstract
Abstract The genetic diversity of Elymus dahuricus in four Northwest Chinese provinces was evaluated using morphological and simple repeat markers. Eighty-six phenotypic traits were investigated. Significant differences between different ecotypes and significant genetic diversity were observed. The coefficient of variation for 8 phenotypic traits ranged from 14.27% to 66.67%, and the F-test indicated that these 8 morphological traits were significantly different between different samples (p < 0.05). Correlation analysis indicated that there were different degrees of connection between the phenotypic traits. A cluster analysis dendrogram using the European distance (15) as separation criteria for the samples was grouped into three categories. A total of 40 bands were screened from 50 pairs of primers, of which 32 bands were polymorphic. A total of 6.4 bands were amplified using each pair of primers. The polymorphic bands from each primer pair were amplified from 4 to 9, and the percentage of polymorphisms was between 67% and 90%. The average percentage of polymorphisms was 80%. The genetic distance of the 16 tested samples ranged from 0 to 9.407, and the genetic similarity coefficients ranged from 0.375 to 1.000, with an average value of 0.686. These results will contribute to the collection, conservation and future genetic improvement of this species.
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