PJB-2021-483
PRE-SOWING SEED TREATMENT WITH PROLINE, TYROSINE AND POTASSIUM NITRATE (KNO3) INCREASE SALT STRESS TOLERANCE IN WHEAT
Sonia Naz
Abstract
To explore the effect of pre-sowing seed treatment with proline, tyrosine, potassium nitrate and mixture of these compounds on two wheat (Triticum aestivum L.) varieties (var. Galaxy-2013 and var. Anaj-2017) a pot experiment was conducted. There were five treatment levels i.e., control (non-primed) and priming with proline (1 mM), tyrosine (1 mM), KNO3 (1 mM) and mixture of proline, tyrosine and KNO3 in 1:1:1 ratio under two levels of salt stress such as control (0 mM NaCl) and salt stress (100 mM NaCl in full strength Hoagland’s nutrient solution). Data of seven week old wheat plants was collected for the determination of various morphological and physio-biochemical parameters of wheat plants. Salt stress (100 mM NaCl) significantly decreased growth, yield, relative water contents (%) and chlorophyll contents but significantly increased chlorophyll a/b, membrane permeability (%), total phenolics, melondialdehyde (MDA), hydrogen peroxide (H2O2), total soluble proteins, total free amino acids, free proline, glycinebetaine and antioxidant enzymes activities. Pre-sowing seed treatment with proline, tyrosine, KNO3 and their mixture significantly enhanced growth, yield and biochemical attributes under salt stress. There was significant difference between two wheat varieties under salt stress. Of all, pre-sowing seed treatments with proline (1 mM) and KNO3 (1 mM) significantly modulated most of the measured growth and yield attributes. Tyrosine treatment (1 mM) exhibited significant impact on growth attributes. Moreover, var. Anaj-2017 showed significant effect in all other measured parameters and proved tolerant under salt stress. Keywords: Anaj-2017, glycinebetaine, antioxidant enzymes activities, total soluble proteins
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