PJB-2021-477
OPTIMIZING THE PRODUCTION OF XYLANASE BY ASPERGILLUS FLAVUS ZGCL17 USING WASTE FIBER SLUDGE
BUSHRA KALIM
Abstract
Waste fiber sludge (WFS) and wheat bran (WB) were used as a substrate for xylanase production by Aspergillus flavus ZGCL17 isolated from fertile soil. The substrates were washed, dried, milled and wetted with Vogel’s medium. Cultures were grown at 31 °C for 11 days. Cell free culture filtrate (CFCF) was subjected to enzyme activity estimation. Fermentation conditions were optimized for xylanase production observing the effects of time course, temperature, pH, substrate concentration, carbon and nitrogen supplements. The optimum culture conditions for the xylanase production by Aspergillus flavus ZGCL17 were observed to be on the 7th day of incubation at 30 °C and pH 5 with 4% substrate concentration. Aspergillus flavus ZGCL17 produced significant amounts of xylanase under submerged fermentation, which may have potential in industrial applications.
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