PJB-2021-455
ISOLATION AND IDENTIFICATION OF PHYLLOSPHERE BACTERIA FROM THREE DIFFERENT CROPS
N Ravimannan
Abstract
Phyllosphere represents the habitat provided by the above ground parts of plants. There have been few researches done on phyllosphere of plants. Large number of microbes associates with the phyllosphere where the most dominant are the bacteria. The main objective of the present study is to isolate phyllosphere bacteria from three different crops cultivated in the Jaffna district. Phyllosphere bacteria were isolated by using washings of host leaf and plating on nutrient agar medium. Two different phyllosphere bacteria namely Pseudomonas putida, Staphylococcus equorum and two different species of the genus Bacillus (B.cereus and B.pumilus) were isolated from Zea mays. Staphyllococcus epidermis and two different species of Bacillus (B.cereus and B.subtilis) were isolated from Solanum lycopersicum. Two different phyllosphere bacteria namely Lactobacillus brevis, Micrococcus luteus and three different species of Bacillus (B.subtilis, B.mycoides and B.licheniformis) were isolated from Brassica oleracea var.capitata.. The isolates were identified based on the colony morphological characteristics and biochemical tests. Keywords Phyllosphere, Bacteria, Zea mays, Solanum lycopersicum, Brassica oleracea var capitata
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