PJB-2021-452
Characterization of Advanced Lines of Cotton (Gossypium Hirsutum L.) through Physio-Biochemical Approaches
Shahwar Fatima
Abstract
This study was conducted to find an advanced line of cotton which has tolerance to cotton leaf curl virus disease and have better composition of leaf physiological and biochemical composition. The experiment was conducted at Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad. The experimental material consists of six advanced lines of cotton NIAB-4-100-5, NIAB-4-79-2, NIAB-4-54, NIAB-1-1, NIAB-14-43-19 and NAIB-4-44-3 compared with standard varieties FH-Lalazar and MNH-886. The experiment was done in a randomized complete block design (RCBD) with three replications. Activities of the anti-oxidative enzymes were measured in the top 4th leaf of cotton genotypes growing in the field. Samples were collected when white fly occurrence was at peak and CLCuV appear. The cotton leaf curl virus (CLCuV) disease prevalence was non-significant among all the genotypes. The maximum grade 3 was observed in NIAB-1-1 which was non-significantly different with NIAB-4-54 and MNH-886. The minimum value 1.53 was observed in FH-Lalazar which was non-significantly different with NIAB-4-100-5, NIAB-4-79-2, NIAB-14-43-19 and NIAB-4-44-3. Maximum yield (257.4g) was recorded in CLCV resistant genotype (NIAB-4-44-3) and minimum yield (190.58g) was observed in susceptible genotype (NIAB-4-54). In the present study, the total phenols (TP), total soluble proteins (TSP), ascorbate peroxidase (APX), peroxidase (POD), catalase (CAT), were studied in cotton leaves. The results showed that phenolic content, total soluble proteins, POX, CAT, and APX played dynamic role in resistance against CLCuV. The amount of TP and TSP were significantly higher in leaves of /tolerantresistant genotypes. Among eight genotypes, the TSP content were maximum in standard variety FH-Lalazar followed by NIAB-4-44-3. All genotypes show highly significant (p<0.01) differences in total phenolic (TP) contents. Results revealed significant differences among the genotypes for enzyme activities. Inconsistent differential behavior was observed among cotton genotypes.
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