PJB-2020-492
LABORATORY CULTURE OF ULVA INTESTINALIS UNDER DIFFERENT SALINITY, TEMPERATURE AND ILLUMINANCE
Saba Rizwan
Abstract
The reproductive pattern of green seaweed Ulva intestinalis was studied from 14th November to 28th November 2014 , and the reproduction of U. intestinalis were observed under the conditions with different salinities 20, 25, 30 ppt, 20, 40 µmol m-2 s-1 light condition, ,and different water temperatures 10 and 20oC. Asexual and vegetative phase of reproduction observed during the fourteen days of culture supplemented with 1mol/L NaNO3 and 0.1 mol/L KH2PO4 seawater. No sexual reproduction was observed, but gametes observed in 10 oC, 20 µmol m-2 s-1 and 20 ppt salinity respectively. The maximum SGR shown in (40 µmol m-2 s-1, 25 ‰ and 20 oC) 27.56% SGR with increased length of filament 11.93±6.64 mm. Proliferation of U. intestinalis thallus could vegetatively grow. The maximum size observed is 55.89±34.49 µm when cultured in 25 ppt, 20 µmol m-2 s-1 and 10oC respectively. During the asexual reproduction, the motile male and female zoospores was observed in 20-40 µmol m-2 s-1,10-20 oC, 20 and 25 ppt cultured medium. It is indicated that U. intestinalis grow fastly by vegetatively and asexually in all trial of culture experiment.
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