PJB-2020-488
ITRAQ-BASED PROTEOMIC ANALYSIS OF THE RESPONSE TO RALSTONIA SOLANACEARUM IN POTATO
JINLIN FENG
Abstract
Bacterial wilt is a serious disease of potato (Solanum tuberosum L.) caused by the soil-borne pathogenic bacterium Ralstonia solanacearum. Detecting changes in protein abundance in potato plants in response to R. solanacearum is a pivotal step in uncovering the molecular interactions of plant pathogens. In this study, using the disease-resistant cultivar ‘Zhongshu 3’, we analyzed protein expression in potato seedlings inoculated with R. solanacearum every 12 h for a total of 72 h using isobaric tags for relative and absolute quantitation-based proteomics. Our results indicate that pathogenesis-related proteins, stress-related proteins, non-specific lipid transfer proteins, small heat shock proteins, and osmotin-like proteins were up-regulated in response to pathogen infection at different time points. The accumulation of these proteins in response to biotic stress suggests that these proteins play an important role in pathogen resistance. Our findings will provide an important basis for characterizing the role of these proteins in increasing plant resistance to pathogens and in breeding bacterial wilt-resistant plants.
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