PJB-2020-473
IN VITRO ANTIMICROBIAL AND DETOXIFICATION EFFECTS OF THE METHANOLIC EXTRACT OF EUPHORBIA DRACUNCULOIDES LEAVES ON HYDROGEN PEROXIDE-INDUCED OXIDATIVE STRESS IN HUMAN LYMPHOCYTES
MUHAMMAD ALAM ZEB
Abstract
Abstract In the present studies, antimicrobial potential and the detoxifying effect of Euphorbia dracunculoides leaves methanolic extract (EDLME) on human lymphocytes under oxidative stress was investigated. The EDLME was a rich source of natural phenolics (259.767 mg gallic acid equivalents / g of the residue). The EDLME showed appreciable antimicrobial and free radical scavenging activities. The lymphocytes were subjected to oxidative stress by treating them with H2O2 (100 µM). The increasing concentration (1 µg/ml - 100µg/ml) of EDLME exhibited stimulatory effect on antioxidant enzymes in stressed cells. The correlation of ROS was significantly negative with SOD (r=-0.912, p=0.0016), CAT (-0.823, p=0.0119) and POD activity (r=-0.894, p=0.0027). It is inferred that either the extract may contain constituents involved in safe guarding of cell lipids from peroxidation or it is equipped with a phytochemisty involved in enhancing the activity of ROS detoxifying enzymes.
To Cite this article:
Download
