PJB-2020-463
DISCOVERY OF ONE NOVEL NATURAL MUTATION WITH DETERMINATE AND CAPITULUM-LIKE INFLORESCENCES AND SPECULATION OF ITS POTENTIAL REGULATORY NETWORKS BY RNA-SEQUENCING IN BRASSICA NAPUS
WEI WAN
Abstract
Brassica napus is an important vegetable and oil crop worldwide. The research of inflorescence is meaningful for yield and plant architecture in B. napus. In this study, one novel natural mutant (MT) line with determinate and capitulum-like inflorescence and its wild-type (WT) line were chosen for RNA-Sequencing. A comparative transcriptome analysis was done for determining the mechanism of forming the MT phenotype at the whole genome expression level and identifying the candidate genes related to the MT phenotype. A total of 6,309 differentially expressed genes (DEGs) were identified, among which 4,100 ones (65.0%) were down-regulated and 2,209 ones (35.0%) were up-regulated in MT compared with WT. Of those DEGs, a total of 4,548 DEGs were successfully annotated by Gene Ontology (GO, 71.68%) and Kyoto Encyclopedia of Genes and Genomes (KEGG, 33.71%). Through the function annotation, a total of 133 candidate genes for regulating the flower development (75 genes, 56.4%), shoot meristem development (29 genes, 21.8%) and inflorescence meristem development (13 genes, 9.8%) were further identified, and a proposal mechanism involved a complex network for the development of inflorescence and flower was suggested. This study has provided us with novel insights on elucidating the molecular mechanism of specially mutated determinate phenotype, and a solid molecular foundation for the future improvement of plant architecture in B. napus.
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