PJB-2020-456
Phenolic compounds identification, antioxidant activity (in vitro) and acute oral toxicity in wistar rat of the Moroccan Lavandula stoechas plant
Moncef Boufellous
Abstract
Lavandula stoechas, known as "Halhal" vascular is a medicinal plant of the lamiaceae family, widely used in traditional medicine worldwide as an antispasmodic in colic pain, expectorant, stimulant for various diseases of the central nervous system. In the context of the valorization of this plant, two extracts with two preparation methods (maceration and soxhlet) were prepared from the different parts of this plant: one ethanolic and the other dichloromethane. The quantitative estimation of total polyphenols, flavonoids and tannins by the colorimetric method showed that the extracts are rich in these compounds. The evaluation of antioxidant capacity carried out by the DPPH free radical trapping method indicated that the ethanolic extract has significant antioxidant activity in the stem part, with the effective concentration to inhibit the 50% DPPH (EC50) is 0.096 mg/ml, higher than that recorded by ascorbic acid (EC50 = 1.04 µg/ml). The analysis of the ethanolic extract of Lavandula stoechas by GC identified several common compounds between the four parts, with different percentages and retention times, most of which are antioxidants, bicyclic monoterpene derivatives, monounsaturated and polyunsaturated fatty acids, alkanes, sterols and triterpenoids. L-camphor (42.68%), palmitic acid (17.29%), linoleic acid (15.98%) and oleic acid (14.01%) were the main components. The determination of the Lethal dose 50 (LD50) of ethanol extracts showed that the fruit is the most toxic with a value of 3548.1 mg/kg which is significant compared to the other parts.
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