PJB-2020-367
SALT RESPONSE ANALYSIS IN THREE CONTRASTING RICE CULTIVARS (ORYZA SATIVA L.) AT SEEDLING STAGE
TRINH NGOC NAM
Abstract
Salt contamination of soils due to climate change is facing a serious environmental issue affected the crop production today. However, the mechanism of plant cellular and molecular responses to salt stress is not fully understood. The present study investigated biochemical and molecular changes under salt stress in root seedlings of three rice cultivars, i.e. AGPPS114 (salt tolerant), OM6967 (moderately tolerance), VD20 (salt sensitive). Increasing salt concentration leads to reduction in shoot/root length but different level among the cultivars. In contrast, ROS (reactive oxygen species) accumulation and lipid peroxidation increased progressively with increasing salt concentration and time course treatment. However, at 250 μM of NaCl these parameters were more adversely affected in VD20 than AGPPS114 and OM6967. In addition, using ICP indicated that Na+ content in root increased gradually with increasing NaCl concentrations in the treatment in all cultivars, but low in salt tolerant cultivar AGPPS114 as compared to other cultivars. Antioxidant enzyme activity assay showed that superoxide dismutase (SOD) and catalase (CAT) were induced during salt treatment in all cultivars. The results also showed that greater proline and glycinebetaine accumulation in the AGPPS114 as compared to OM6976 and VD20. Real-time PCR indicated the significant difference in transcript levels of Na+-transporter gene OsSOS1, OsNHX1 and OsHKT1s in AGPPS114 and OM6967 cultivars compared to VD20 cultivar. In summary, In summary, the salt tolerant cultivar maintained higher antioxidant defense and ion transporter systems without elevated levels of proline and glycinebetaine accumulation than salt sensitive rice during salt stress at seedling stage. Key words: reactive oxygen species, real-time PCR, rice (Oryza sativa L.), isozyme, salt tolerance, Na+-transporter gene
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