PJB-2020-341
PHYTOCHEMICAL CONSTITUENTS, TOTAL ANTIOXIDANT CAPACITIES AND SKIN PIGMENTATION INHIBITORY ACTIVITIES OF NIGERIAN CANNABIS SATIVA
EMMANUEL ILESANMI ADEYEYE
Abstract
Antioxidant and inhibition of premature skin ageing activities of medicinal plants, in addition to varying degree of health benefits have received global recognition in recent times. The present study investigated the quantification of phytochemicals in the root, leaf, stem, seed, seed plus leaf (SK) of Cannabis sativa. The total antioxidant capacities of the plant extracts in hexane, pet-ether, ethyl acetate, methanol, butanol and water as well as their anti-lipid peroxidation (LPO) and tyrosinase (TYR) inhibitory activities were also investigated in an in-vitro system. The class of compounds therein contained in the respective plant extracts were investigated using assays of phenolics, flavonoid, flavonol and flavanol, while the total antioxidant capacities were determined through evaluation of trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant potential (FRAP) assays. The outcome of this study showed that leaf contained highest flavonoids (532 ± 0.92 mg QE/g), phenolics (264 ± 8.73 mg GAE/g), flavanol (144 ± 2.70 mg CE/g) and flavonol (102 ± 3.78 mg QE/g). Aqueous extract of the leaf was found to contain highest FRAP of 1589 ± 20.4 μM AAE/g greater than the commercial antioxidant Epigallocatechin gallate (EGCG) of 1429 ± 0.82 μM AAE/g. The SK has the highest TEAC of 3172 ± 1.38 μM TE/g also greater than EGCG 2932 ± 9.97 μM TE/g. The seed extract of hexane has the highest LPO value of 89.1 ± 1.55 mg/mL, while the leaf extract of methanol has the highest TYR value of 82.2 ± 4.77 mg/mL. The results showed that the plant extracts can be used to reduce oxidative stress as well as inhibiting deleterious effect of skin enzyme responsible for the formation of pigments on the surface of the skin as well as perishable agricultural products.
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