PJB-2020-339
CRE-MIR914-REGULATED RPL18 ENHANCED SALINITY RESISTANCE IN CHLAMYDOMONAS REINHARDTII
BO WANG
Abstract
Global climate change has many serious consequences and, one of the major problems is the expansion of soil salinization. High salt levels reduce the photosynthetic efficiency of plants by damaging the structure and function of the photosynthetic apparatus, and thereby influencing plant growth and development. Many researches have shown that small RNAs play an essential role in the stress adaptation of eukaryotic cells. In previous study, we have found that Cre-miR914 played a negative role in UV-B radiation and heat stress in Chlamydomonas reinhardtii, but whether Cre-miR914 is also involved in regulation of salt-stress is unknow. Bioinformatics analysis indicated that Cre-miR914 may targets the RPL18 (ribosomal protein L18) gene. In this study, we found Cre-miR914 and RPL18 expression patterns showed an opposite trend under salt stress. Furthermore, we constructed cell lines overexpressing Cre-miR914 or RPL18, and performed survival experiments under salt-stress. The results showed that Cre-miR914 overexpression decreased salt-stress resistance and RPL18 overexpression increased salt-stress tolerance in C. reinhardtii. These results indicate that Cre-miR914 and RPL18 exert a considerable influence on salinity resistance, which may provide a new method for improving salt-stress tolerance in plants.
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