PJB-2020-334
GENETIC DIVERSITY STUDIES AND DNA FINGERPRINTING OF PAKISTANI POTATO VARIETIES USING SIMPLE SEQUENCE REPEAT MARKERS
SAJID UR RAHMAN
Abstract
DNA fingerprinting provides a tool for variety identification, plant breeder rights protection, and variety registration in Plant Breeder Rights Repository. In the present study we developed a DNA fingerprinting profile of 12 potato cultivars grown in Punjab Pakistan using 214 informative SSR markers. Among 214 amplified SSR markers 21 were monomorphic and the remaining 193 were polymorphic. A total of 1720 alleles were amplified by 214 SSR with an average of 8.04 alleles per marker. Approximately 72% of amplified alleles (1329 alleles) were polymorphic with 6.88 polymorphic alleles per SSR marker. The number of alleles ranged from 1 to 31. Similarly, polymorphic alleles per markers ranged from 0 to 24. A maximum number of alleles and polymorphic alleles were reported by SSR marker IBR13. The PIC value ranged from 0 to 0.96. The average PIC value for 214 amplified markers was 0.73. Collectively 72 SSR markers amplified unique allelic patterns for DNA fingerprinting. Potato varieties Rubby and Sadaf were identified by 15 SSR markers whereas Faisalabad Red and SH-5 were identified by 12 SSR markers. Cluster and structure analysis classify the potato genotypes to two distinct groups i.e. Cluster I with five genotypes Faisalabad Red, Faisalabad White, SH-5, Rubby and PRI Red whereas Cluster II was comprised of Sadaf, Sialkot Red, Sahiwal White, Sahiwal Red, Ravi Red, FD 81-1 whereas Cosmo did not obey clustering. The information will be useful for the variety registration process and will provide a platform for future DNA fingerprinting and genetic diversity studies for the choice of SSR markers.
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