PJB-2020-278
EVALUATION OF GENETIC STABILITY OF PLANTLETS FROM TISSUE CULTURE DATE PALM (CV. MEDJOOL) USING SSR MARKERS
ATHENA BESTAGI
Abstract
Date palm (Phoenix dactylifera L.) is a monocotyledonous, dioecious and perennial plant with a long life, which has high economic importance in Iran. The tissue culture method of the date palm, including somatic embryogenesis, can lead to a somaclonal variation that is caused by genetic diversity and epigenetic variation. Therefore, the lack of genetic stability in tissue culture-derived plantlets leads to the unwillingness of the date palm orchardist to use this type of plantlets. In this research, the genetic stability of 4 maternal date palm and 180 in vitro seedlings obtained from somatic embryogenesis was evaluated in the Medjool date cultivar using 24 pairs of SSR primers. None of the primers did succeed to reveal the difference between the tissue culture derived seedlings and maternal plant within a cultivar. The results of this study showed that the date plantlets proliferated by tissue culture method genetically were similar to maternal plants. Therefore, the rapid and inexpensive method for propagation of the Medjool date palm to produce plantlets using in vitro somatic embryogenesis is recommended.
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