PJB-2020-260
Rehmannia glutinosa germplasm genotyping based on single nucleotide polymorphisms
Zhou Yanqing
Abstract
Rehmannia glutinosa is a perennial herb with some important medicinal, edible and ornamental values. Its yield and quality are significantly affected by its genotypes because of germplasm complexity. So far, Rehmannia glutinosa germplasms have been identified by some DNA molecular markers, there is few report on single nucleotide polymorphisms (SNPs) with their high level of polymorphism, favorable reproducibility and wide genomic distribution. In this study, we developed seven polymorphic single nucleotide polymorphisms (SNPs) from Rehmannia glutinosa transcriptome databases, constructed its fingerprinting and identified its germplams. 102075 Unigenes containing 35339 SNP loci were isolated from Rehmannia glutinosa transcriptome reads. Forty SNP loci were randomly selected from 35339 SNP loci for PCR validation using twenty-seven samples as templates. Eight polymorphic SNP loci were screened out of these loci, and used to construct the fingerprinting of twenty seven Rehmannia glutinosa germplasms.Based on this fingerprinting, sixteen different Rehmannia glutinosa germplasms could be identified. These new SNP markers will provide an effective tool for Rehmannia glutinosa germplasms genotyping.
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